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KMID : 1033620200470040284
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Clinical and Experimental Reproductive Medicine
2020 Volume.47 No. 4 p.284 ~ p.292
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Effect of evaporation-induced osmotic changes in culture media in a dry-type incubator on clinical outcomes in in vitro fertilization-embryo transfer cycles
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Chi Hee-Jun
Park Jun-Sang
Yoo Chang-Seok
Kwak Su-Jin
Son Ho-Jeong
Kim Seok-Gi
Sim Chae-Hee
Lee Kyeong-Ho
Koo Deog-Bon
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Abstract
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Objective: This study investigated whether adding outer-well medium to inhibit osmotic changes in culture media in a dry-type incubator improved the clinical outcomes of in vitro fertilization-embryo transfer (IVF-ET) cycles.
Methods: In culture dishes, the osmotic changes in media (20 μL)-covered oil with or without outer-well medium (humid or dry culture conditions, respectively) were compared after 3 days of incubation in a dry-type incubator. One-step (Origio) and G1/G2 (Vitrolife) media were used.
Results: The osmotic changes in the dry culture condition (308 mOsm) were higher than in the humid culture conditions (285?290 mOsm) after 3 days of incubation. In day 3 IVF-ET cycles, although the pregnancy rate did not significantly differ between the dry (46.2%) and humid culture (51.0%) groups, the rates of abortion and ongoing pregnancy were significantly better in the humid culture group (1.5% and 49.5%, respectively) than in the dry culture group (8.3% and 37.8%, respectively, p<0.05). In day 5 IVF-ET cycles, the abortion rate was significantly lower in the humid culture group (2.2%) than in the dry culture group (25.0%, p<0.01), but no statistically significant difference was observed in the rates of clinical and ongoing pregnancy between the dry (50.0% and 25.0%, respectively) and humid culture groups (59.5% and 57.3%, respectively) because of the small number of cycles.
Conclusion: Hyperosmotic changes in media occurred in a dry-type incubator by evaporation, although the medium was covered with oil. These osmotic changes were efficiently inhibited by supplementation of outer-well medium, which resulted in improved pregnancy outcomes.
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KEYWORD
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Clinical outcomes, Dry-type incubator, Evaporation, Osmotic change, Outer-well medium
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